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Trichoderma enzymes promote Fibrobacter succinogenes S85 adhesion to, and degradation of, complex substrates but not pure cellulose
Author(s) -
Morgavi Diego P,
Beauchemin Karen A,
Nsereko Victor L,
Rode Lyle M,
McAllister Tim A,
Wang Yuxy
Publication year - 2004
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.1790
Subject(s) - fibrobacter succinogenes , cellulose , rumen , cellulase , silage , food science , chemistry , hay , adhesion , substrate (aquarium) , bacteria , biochemistry , biology , zoology , fermentation , organic chemistry , ecology , genetics
Abstract The effects of an enzyme preparation from Trichoderma longibrachiatum (TE) on adhesion and growth of the fibrolytic rumen bacterium Fibrobacter succinogenes S85 was studied to gain a better understanding of the action of feed enzyme additives on fibre digestion by ruminants. Adhesion experiments were performed on crystalline cellulose, corn silage and alfalfa hay. Adhesion of F succinogenes to cellulose was negatively related to the concentration of TE ( p < 0.05). At the highest concentration used, TE reduced adhesion to cellulose from 65 to 39%. For corn silage and alfalfa hay, TE stimulated adhesion at low levels ( p < 0.05) but this effect was lost at higher levels. Culture experiments were performed on crystalline cellulose and corn silage. The presence of TE in media containing cellulose failed to increase substrate disappearance or gas production although it increased numbers of non‐adherent bacteria ( p < 0.05). When corn silage was used, the addition of TE increased NDF disappearance ( p < 0.05) at 24 and 48 h (33 and 52% in controls versus 53 and 65% in TE treatments). Growth rate and gas production were also stimulated ( p < 0.05). We conclude that, for cellulose, the hydrolytic enzymes in TE obstructed available binding sites decreasing bacterial adherence. Fibrobacter succinogenes digested cellulose efficiently and addition of exogenous cellulases did not further increase substrate disappearance. However, for complex plant substrates, low concentration of TE increased bacterial adhesion and plant (corn) fiber degradation. For the Department of Agriculture and Agri‐Food, Government of Canada, © Minister of Public Works and Government Services Canada 2004. Published for SCI by John Wiley & Sons, Ltd.