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A survey of the fungal contamination and presence of ochratoxin A and zearalenone on Spanish feed and raw materials
Author(s) -
Jaimez Judith,
Fente Cristina A,
Franco Carlos M,
Cepeda Alberto,
Vázquez Beatriz I
Publication year - 2004
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.1763
Subject(s) - zearalenone , ochratoxin a , mycotoxin , contamination , fusarium , food science , food contaminant , aflatoxin , raw material , biology , chemistry , microbiology and biotechnology , toxicology , horticulture , ecology
The incidence of fungal contamination in 91 samples of feed and raw materials used for animal feeding in Spain has been studied. Sample analysis was accomplished in a new culture medium to which a beta‐cyclodextrin had been added, and a comparison with other more usual culture media was performed. Ochratoxin A (OTA) and zearalenone (ZEA) contamination of all the samples was evaluated by RP‐HPLC with fluorescence detection. The fungal genera found, such as Penicillium and Fusarium , included mycotoxigenic strains as OTA and ZEA (33.3 and 26.4% incidence respectively). One sample of corn and another of cotton seed were contaminated with levels of OTA above the 5 and/or 10 µg kg −1 recommended by the legislation of several European countries, whereas none of the samples contaminated with ZEA surpassed the legislation limits suggested by the official agencies. Copyright © 2004 Society of Chemical Industry