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Determination of fat in live farmed Atlantic salmon using non‐invasive NIR techniques
Author(s) -
Solberg Christel,
Saugen Esten,
Swenson LarsPetter,
Bruun Lars,
Isaksson Tomas
Publication year - 2003
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.1363
Subject(s) - monochromator , partial least squares regression , calibration , wavelength , near infrared spectroscopy , correlation coefficient , environmental science , optics , zoology , analytical chemistry (journal) , materials science , mathematics , chemistry , biology , optoelectronics , statistics , chromatography , physics
Non‐destructive near‐infrared (NIR) measurements were performed on 100 live, anaesthetised farmed Atlantic salmon, whole weight 1–11 kg, using two different NIR instruments: a grating monochromator instrument equipped with a fibre optic interactance probe, and a diode array instrument measuring diffuse reflectance in a non‐contact mode. Crude fat content was determined using partial least squares (PLS) regression. Full cross‐validation was used to evaluate the performance of the calibration models, expressed as the root mean square error of prediction (RMSEP). For the fibre optic instrument the wavelength range from 800 to 1098 nm resulted in a correlation coefficient of 0.90 and an RMSEP equal to 14 g kg −1 fat. The diode array instrument using the wavelength range from 900 to 1700 nm gave results of the same accuracy. The measurement times were 21 and 3 s respectively. It is concluded that either instrument could be used to determine the crude fat content in live Atlantic salmon, with good accuracy. © 2003 Society of Chemical Industry