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The diurnal pattern of ileal dry matter and endogenous ileal nitrogen flows in the growing pig
Author(s) -
Hodgkinson Suzanne M,
Moughan Paul J,
Morel Patrick C H,
Reynolds Gordon W
Publication year - 2002
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.1258
Subject(s) - endogeny , dry matter , ileum , zoology , reabsorption , chemistry , digestion (alchemy) , cecum , biology , medicine , endocrinology , chromatography , kidney , ecology
The aim was to examine the diurnal pattern of endogenous nitrogen flow at the terminal ileum of the pig. Seven entire male pigs with a mean body weight of 58 kg had post‐valve T‐caecum (PVTC) cannulae surgically implanted for the collection of ileal digesta. The pigs were fed equal‐sized meals (10% of metabolic body weight, W 0.75 , per day) twice daily at 08:00 and 17:00. The semi‐synthetic diet included enzyme‐hydrolysed casein (<5000 Da, 100 g kg −1 diet) as the sole source of nitrogen (N). The diet also contained chromic oxide (6 g kg −1 ) as an indigestible marker. The pigs received the diet for an 8 day period, and digesta were continuously collected for 24 h periods (commencing at 08:00) on the fifth and eighth days. Digesta were analysed for dry matter (DM) and chromium (Cr), and endogenous N was determined in the precipitate + retentate fraction of the digesta following centrifugation and ultrafiltration (10 000 Da molecular weight cut‐off). The concentration of Cr expressed on a digesta DM basis and the ratio of endogenous N to Cr in the digesta were relatively constant over the 24 h digesta collection periods, with no statistically significant ( P > 0.05) differences found for the latter measure in the digesta collected between 12:00 and 08:00. The endogenous N flow through the terminal ileum is the net effect of the secretion and reabsorption of endogenous N that occurs throughout the digestive tract. The net result of the latter two processes in the small intestine of the pig leads to a relatively constant endogenous N flow over time post‐feeding. © 2002 Society of Chemical Industry