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Efficient biorefinery of whole cassava for citrate production using Aspergillus niger mutated by atmospheric and room temperature plasma and enhanced co‐saccharification strategy
Author(s) -
Wang Baoshi,
Tan Fengling,
Yu Feifei,
Li Hua,
Zhang Mingxia
Publication year - 2021
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.11104
Subject(s) - biorefinery , aspergillus niger , fermentation , citric acid , phytase , hydrolysis , food science , cellulase , microbiology and biotechnology , chemistry , sugar , pulp and paper industry , corn steep liquor , biochemistry , biology , enzyme , biofuel , engineering
Abstract BACKGROUND The non‐grain crop cassava has attracted intense attention in the biorefinery process. However, efficient biorefinery of whole cassava is faced with some challenges due to the existence of strain inhibition and refractory cellulose during the citrate production process. RESULTS Here, a novel breeding method – atmospheric and room temperature plasma (ARTP) – was applied for strain improvement of citrate‐producing strain Aspergillus niger from whole cassava. The citrate yield of the mutant obtained using ARTP mutagenesis increased by 36.5% in comparison with the original strain. Moreover, citric acid fermentation was further improved on the basis of an enhanced co‐saccharification strategy by supplementing glucoamylase and cellulase. The fermentation efficiency increased by 35.8% with a 17.0 g L −1 reduction in residual sugar on a pilot scale. CONCLUSIONS All these results confirmed that a combination of the novel breeding method and enhanced co‐saccharification strategy could be used to efficiently refine whole cassava. The results also provide inspiration for the production of value‐added products and waste disposal in agro‐based industries. © 2021 Society of Chemical Industry