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Quantification of tea‐derived catechins without the requirement for respective calibration curves by single reference liquid chromatography based on relative molar sensitivity
Author(s) -
Takahashi Miki,
Nishizaki Yuzo,
Masumoto Naoko,
Sugimoto Naoki,
Sato Kyoko,
Inoue Koichi
Publication year - 2021
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.11013
Subject(s) - chromatography , chemistry , calibration curve , calibration , trimethylsilyl , flavonoid , analytical chemistry (journal) , green tea , detection limit , mathematics , organic chemistry , food science , statistics , antioxidant
BACKGROUND Many studies report the monitoring of catechins in tea samples by chromatographic techniques. Unfortunately, only a small number of screening assays for catechins exist as a result of the complexity of authentic standards for the respective calibration curves. In the present study, a single reference (SR) exhaustive assay for the simultaneous quantification of tea‐derived catechins by liquid chromatography (LC) with photodiode array and fluorescence detectors based on relative molar sensitivity (RMS) was developed as a screening assay of common tea samples without respective calibration curves using authentic standards. RESULTS Three original SR standards were proposed based on flavonoid structures, evaluated by quantitative 1 H‐NMR based on an indirect standard (1,4‐bis(trimethylsilyl) benzene‐ d 4 ) and successfully separated in a LC chromatogram. In tea samples with these added SR calculated based on RMS, the concentrations of eight tea‐derived catechins could be measured with a relative SD of < 8.5% by a single LC run. CONCLUSION This LC screening assay based on RMS allows reliable quantification without the requirement for respective calibration curves using authentic standards. © 2020 Society of Chemical Industry