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Sea buckthorn ( Hippophae rhamnoides L.) fermentation liquid protects against alcoholic liver disease linked to regulation of liver metabolome and the abundance of gut microbiota
Author(s) -
Ran Beibei,
Guo ChangE,
Li Weidong,
Li Weishi,
Wang Qing,
Qian Jinxiu,
Li Hailong
Publication year - 2021
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10915
Subject(s) - gut flora , biology , liver injury , akkermansia , alcoholic liver disease , fatty liver , triglyceride , superoxide dismutase , prebiotic , oxidative stress , microbiology and biotechnology , lactobacillus , biochemistry , cholesterol , medicine , fermentation , endocrinology , cirrhosis , disease
BACKGROUND Alcoholic liver disease (ALD) refers to liver damage caused by long‐term heavy drinking, which causes oxidative stress and changes in gut microbiota. In this paper, we investigated the hepatoprotective effect of sea buckthorn fermentation liquid on ALD in mice and the interaction between ALD and gut microbiota using animal experiments and gut microbiota measurements. RESULTS We found that the contents of total flavonoids, total triterpenes and related short‐chain fatty acids (SCFAs) in sea buckthorn fermentation liquid (SFL) were significantly greater. Liver index, kidney index, spleen index, serum indexes of liver injury – alanine aminotransferase (ALT) and spartate aminotransferase (AST), inflammatory factors in liver tissues – tumor necrosis factor‐ α (TNF‐ α ) and interleukin‐6 (IL‐6), oxidation indexes – malondialdehyde (MDA) and superoxide dismutase (SOD), and lipid metabolism indexes – high‐density liptein cholesterol (HDL‐C), low density lipoprotein cholesterol (LDL‐C), and triglyceride (TG), suggested that SFL significantly ameliorates liver injury caused by alcohol. By measuring gut microbiota in mice feces samples, we found that the high‐dose group of SFL reversed the declining trend of the gut microbiota Firmicutes/Bacteroidetes (F/B) ratio caused by alcohol, reducing the number of gram‐negative bacteroidetes. Patescibacteria was tightly connected with the indicators of ALD. At the genus level, high‐dose SFL significantly downregulated Akkermansia , Turicibacter , Alistipes and Ruminiclostridium , and improved the abundance of beneficial bacteria in Lactobacillus . In addition, Alistipes and Ruminiclostridium was closely connected with the indicators of ALD. CONCLUSION Sea buckthorn fermentation liquid protected against alcoholic liver disease and modulated the composition of gut microbiota. © 2020 Society of Chemical Industry