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Structural characterization of calcium‐binding sunflower seed and peanut peptides and enhanced calcium transport by calcium complexes in Caco‐2 cells
Author(s) -
Bao Xiaolan,
Yuan Xingyu,
Feng Guoxue,
Zhang Meili,
Ma Sarina
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10800
Subject(s) - chemistry , sunflower , peptide , calcium , amino acid , biochemistry , helianthus annuus , molecular mass , chromatography , electrospray ionization , ion chromatography , mass spectrometry , biology , enzyme , organic chemistry , agronomy
BACKGROUND Peptide–Ca complexes can promote Ca absorption. The present study aimed to determine the transport mechanism and structural characteristics of sunflower seed and peanut peptides with high Ca binding capacity with respect to developing third‐generation Ca supplements and functional food ingredients. RESULTS High Ca‐binding fractions of 1–3 kDa sunflower seed peptide (SSP 4 ) and ≥ 10 kDa peanut peptide (PP 1 ) had higher amount of Ca transported than CaCl 2 and two hydrolyzed proteins in Caco‐2 cells. SSP 4 and PP 1 were separated by Ca ion metal chelate affinity chromatography, and high Ca‐binding fractions were observed for SSP 4 ‐P 2 and PP 1 ‐P 2 . The amino acid sequences of SSP 4 ‐P 2 and PP 1 ‐P 2 were characterized by high‐performance liquid chromatography‐electrospray ionization‐time of flight mass spectrometry. Seven and eight peptides were identified from SSP 4 ‐P 2 and PP 1 ‐P 2 , respectively. These peptides had molecular weights ranging from 1500 Da to 2500 Da and a large number of characteristic amino acid sequences, such as EEEQQQ, EQ–QQQ–QQ, QQ–, E–EEE, EE–EEQ, RR, Q–QQ–QQQ, EE–EQ–EE–Q, QQ–QQQQ, and Q–QQQQ, where ‘E’ is glutamic acid and ‘Q’ is glutamine. CONCLUSION SSP 4 and PP 1 can promote Ca transport in Caco‐2 cells without affecting cell permeability. The amino acid sequences of SSP 4 ‐P 2 and PP 1 ‐P 2 with high Ca‐binding abilities contain characteristic sequences, such as continuous glutamic acid and glutamine, and have low molecular weights. © 2020 Society of Chemical Industry