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Development and validation of an HPLC‐DAD method for determination of oleuropein and other bioactive compounds in olive leaf by‐products
Author(s) -
MartínezNavarro Esther M,
CebriánTarancón Cristina,
MoratallaLópez Natalia,
Lorenzo Cándida,
Alonso Gonzalo L,
Salinas Rosario M
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10758
Subject(s) - oleuropein , chromatography , high performance liquid chromatography , chemistry , olive leaf , extraction (chemistry) , olea , hydroxytyrosol , chromatography detector , aqueous solution , detection limit , olive oil , polyphenol , food science , botany , antioxidant , biochemistry , organic chemistry , biology
BACKGROUND Oil mills could benefit by preparing their own aqueous extracts from olive leaves. Accordingly, the present study aimed to measure the bioactive compounds richness of such extracts, especially oleuropein. A water‐based microwave extraction procedure was developed and a selective and precise high‐performance liquid chromatography with diode array detection (HPLC‐DAD) method was validated for the determination of oleuropein and others bioactive compounds from olive leaves. RESULTS The water solubility of oleuropein was determined to be 9.5 g L −1 . The extraction procedure was optimized in terms of power, olive leaf weight/water volume ratio and time of extraction, and the results revealed that 2 mg mL −1 and a microwave irradiation at 800 W for 30 s resulted in the greatest efficiency. Oleuropein was determined by the new validation method, which showed good linearity ( r 2 = 0.996), precision (% relative standard deviation < 10%), recovery (118.6%), and limits of detection (17.48 mg L −1 ) and quantification (21.54 mg L −1 ). Good correlation ( r 2 = 0.979) was obtained between oleuropein of the olive leaf extracts determined by HPLC‐DAD and by UV‐visible spectrophotometry. CONCLUSION A simple extraction method was developed and validated to obtain aqueous extract from olive leaves by microwave extraction, determining for the first time oleuropein water solubility. Validation of the method showed that oleuropein in olive leaves could be quantified when it is at least 1% of dry weight by means of HPLC‐DAD. UV‐visible spectrophotometry can be useful in oil mills because it enables the content of oleuropein and other bioactive compounds content to be determined in situ in such leaf aqueous extracts. © 2020 Society of Chemical Industry