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Astilbin lowers the effective caffeine dose for decreasing lipid accumulation via activating AMPK in high‐fat diet‐induced obese mice
Author(s) -
Yang Licong,
Zhu Yanping,
Zhong Shusheng,
Zheng Guodong
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10669
Subject(s) - caffeine , ampk , lipolysis , endocrinology , medicine , chemistry , amp activated protein kinase , protein kinase a , pharmacology , adipose tissue , biochemistry , kinase
BACKGROUND Caffeine has an anti‐obesity effect, although chronic excessive caffeine consumption also causes caffeinism, which is marked by increased anxiety or depression, amongst other symptoms. The present study aimed to investigate whether the addition of flavonoids such as astilbin can reduce the caffeine dose needed to inhibit obesity. RESULTS ICR mice ( n = 80) were fed with normal diet, high‐fat diet (HFD), HFD supplemented with astilbin, caffeine, or astilbin + caffeine for 12 weeks. When diets supplemented with astilbin, 0.3 g kg −1 diet caffeine had the same effect as 0.6 g kg −1 diet caffeine alone, and 0.6 g kg −1 diet caffeine combined with astilbin most effectively inhibited HFD‐induced obesity. Astilbin improved the anti‐obesity effects of caffeine on lipid accumulation via the activation of AMP‐activated protein kinase α (AMPK α ). (i) Activated AMPKα decreased lipid biosynthesis by suppressing the activity or mRNA expression of 3‐hydroxy‐3‐methylglutaryl‐CoA reductase, sterol regulatory element binding protein 1c and its target gene fatty acid synthase. (ii) Activated AMPK α also up‐regulated lipolysis by enhancing the expression of adipose triglyceride lipase and increasing the phosphorylation of hormone‐sensitive lipase. (iii) Finally, activated AMPK α increased carnitine acyltransferase and acyl‐CoA oxidase activities, which further promoted fatty acid β ‐oxidation. CONCLUSION The results obtained in the present study indicate that astilbin may decrease the effective dose of caffeine needed for an anti‐obesity effect and also suggest that it suppresses fat accumulation via the activation of AMPK. © 2020 Society of Chemical Industry