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Effect of pH on properties of golden pompano skin collagen‐based fibril gels by self‐assembly in vitro
Author(s) -
Shi Linfan,
Tian Haohao,
Wang Yanxia,
Hao Gengxin,
Chen Jun,
Weng Wuyin
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10539
Subject(s) - fibril , chemistry , rheology , in vitro , dynamic mechanical analysis , endothermic process , biophysics , collagen fibril , viscoelasticity , polymer , chemical engineering , polymer chemistry , materials science , biochemistry , organic chemistry , composite material , adsorption , engineering , biology
BACKGROUND Application of fish skin collagen has received increasing attention due to mammalian derived diseases and religious limitations. Collagen fibril gel could be formed in vitro through the self‐assembly process. The present study investigated the effect of pH on the self‐assembly in vitro of acid‐solubilized collagen (ASC) from golden pompano skin by determining the turbidity, rheological viscoelasticity, network structure, gel strength, and thermal stability of collagen fibril gel. RESULTS The isoelectric point of ASC was pH 5.27. The turbidity–time and rheological viscoelasticity results indicate that the collagen self‐assembly rate in vitro at pH 7.0 was the slowest. The rate was accelerated by increasing or decreasing the pH. Scanning electron microscopy images show that the fibril diameters of the collagen fibril gels and the collagenous fibril number in the collagen fiber increased with the pH. The gel strength of the collagen fibril gel prepared at pH 5.0 was 22.06 g and increased up to 220.46 g when pH increased to 8.0. No obvious peaks were observed in the differential scanning calorimetry curves of the collagen fibril gels prepared at pH 5.0, whereas high endothermic peak temperature ( T m ) and enthalpy change ( ΔH ) were found in the collagen fibril gels prepared at pH 6.0–8.0. CONCLUSION It is concluded that the physical properties of ASC fibril gels can be improved by increasing the fibril diameter controlled by pH. © 2020 Society of Chemical Industry