Premium
Recovery of value‐added glycopeptides from edible bird's nest ( EBN ) co‐products: enzymatic hydrolysis, physicochemical characteristics and bioactivity
Author(s) -
Ling Jin Wei Alvin,
Chang Lee Sin,
Babji Abdul Salam,
Lim Seng Joe
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10530
Subject(s) - hydrolysate , chemistry , food science , enzymatic hydrolysis , hydrolysis , chromatography , biochemistry
BACKGROUND Processing of edible bird's nest (EBN) requires extensive washing to remove impurities and produces huge amounts of EBN co‐products, which contain mainly feathers with glycoproteins attached, which are usually discarded. This study was conducted to recover the valuable EBN glycoproteins from the waste material. Enzymatic hydrolysis was applied to recover EBN glycopeptides from EBN co‐products (EBN coP ) and processed cleaned EBN (EBN clean ) was used as control, which were then freeze‐dried into EBN hydrolysates (EBNh coP and EBNh clean , respectively). RESULTS The recovery yield for EBNh clean and EBNh coP were 89.09 ± 0.01% and 47.64 ± 0.26%, respectively, indicating nearly 50% of glycopeptide can be recovered from the waste material. Meanwhile, N ‐acetylneuraminic acid, a major acid sugar in EBN glycoproteins, of EBNh coP increased by 229% from 58.6 ± 3.9 to 192.9 ± 3.1 g kg −1 , indicating the enzymatic hydrolysis removed impurities and thus enhanced the N ‐acetylneuraminic acid content. Total soluble protein was more than 330 g kg −1 for all the samples. Colour parameter showed that hydrolysate samples have greater L * (lightness) values. Chroma result indicates the intensity of all the samples were low (< 11). Fourier‐transform infrared (FTIR) spectrum displayed that the EBNh coP exhibited similar functional groups with EBNh clean , indicating that the EBN coP has similar functionality as EBN clean . Significantly higher ( P ≤ 0.05) 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) activities were reported in EBNh coP after the enzymatic reaction. CONCLUSION EBNh coP were successfully recovered from low value EBN coP with enhanced antioxidant activities. The findings of this work are beneficial for the EBN industry to reduce wastage and enhance economic values of EBN co‐products, both economically and nutritionally. © 2020 Society of Chemical Industry