Premium
Regulation by fungal endophyte of Rhodiola crenulata from enzyme genes to metabolites based on combination of transcriptome and metabolome
Author(s) -
Cui JinLong,
Gao XiaoYin,
Vijayakumar Vinod,
Guo ZhangXuan,
Wang MengLiang,
Wang JunHong,
Liu Lei
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10489
Subject(s) - metabolome , metabolite , biology , transcriptome , biochemistry , metabolomics , metabolic pathway , rhodiola , enzyme , metabolism , endophyte , acetaldehyde , gene , botany , gene expression , salidroside , pharmacology , bioinformatics , ethanol
BACKGROUND The contents of some its crucial metabolites tend to decrease when Rhodiola crenulata is cultured at low altitude. Interestingly, it was found that an endophyte, Phialocephala fortinii , could alleviate this problem. RESULTS There were 16 151 differential genes including 14 706 up‐regulated and 1445 down‐regulated unigenes with significant differences ( P < 0.05), and a total of 1432 metabolites exhibited statistically significant ( P < 0.05) metabolic differences comprising 27 different marker metabolites which showed highly significant values of VIP > 5 and P < 0.01. Results highlight differential regulation of 20 enzymatic genes that are involved in the biosynthesis of five different marker metabolites including acetaldehyde, homocysteine, cyclopropylamine, 1‐pyrrolinium and halistanol sulfate. CONCLUSIONS The positive physiological effect of P. fortinii on R. crenulata encompasses differential regulation in carbohydrate metabolism, lipid metabolism and secondary metabolite synthesis. © 2020 Society of Chemical Industry