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Screening antagonistic yeasts against citrus green mold and the possible biocontrol mechanisms of Pichia galeiformis ( BAF03 )
Author(s) -
Chen Ou,
Yi Lanhua,
Deng Lili,
Ruan Changqing,
Zeng Kaifang
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10407
Subject(s) - penicillium digitatum , postharvest , yeast , fungicide , biology , biological pest control , pichia , mold , colletotrichum , blue mold , botany , horticulture , microbiology and biotechnology , food science , biochemistry , penicillium expansum , pichia pastoris , recombinant dna , gene
BACKGROUND Penicillium digitatum is one of the most important pathogens causing citrus green mold, leading to significant economic losses. Traditionally, synthetic fungicides are used to control diseases. However, the side effects of fungicides should not be ignored. Thus, antagonistic yeasts were proposed to be safe and effective alternatives for managing diseases. Orchards are excellent sources of naturally occurring antagonists against pathogens. Therefore, in the present study, antagonistic yeasts obtained from orchards were screened, and the possible biocontrol mechanisms of the most promising yeast were investigated. RESULTS Seventy‐eight isolates of yeasts (15 species of 10 genera) were obtained from citrus orchards. In in vitro assays, 16 strains showed antifungal activity against Pichia digitatum and 15 strains showed biocontrol potential against green mold on Olinda oranges. Pichia galeiformis (BAF03) exhibited the best antagonistic activity against P. digitatum during 6 days storage at 25 °C and a good antagonistic activity during 29 days at 4 °C. Pichia galeiformis (BAF03) could colonize and amplify quickly in wounded citrus. Scanning electron microscopy results showed that the citrus wound was colonised by the yeast. A total of eight volatile organic compounds (VOCs) were identified by gas chromatography‐mass spectrometry The VOCs produced by P. galeiformis (BAF03) efficiently inhibited P. digitatum . CONCLUSION Pichia galeiformis (BAF03) isolated from a citrus orchard showed potential to control postharvest green mold of citrus. The possible mechanisms of action likely include competition for space and nutrients as well as production of VOCs.