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Root colonization by heavy metal resistant Enterobacter and its influence on metal induced oxidative stress on Cajanus cajan
Author(s) -
Sharma Rakesh K,
Barot Kavita,
Archana Gayatri
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10161
Subject(s) - cajanus , rhizobium , bacteria , enterobacter , legume , siderophore , oxidative stress , biology , root nodule , cadmium , root hair , chemistry , colonization , metal toxicity , botany , microbiology and biotechnology , horticulture , biochemistry , symbiosis , escherichia coli , genetics , organic chemistry , gene
BACKGROUND Heavy metal resistant bacterium Enterobacter sp. C1D was evaluated for cadmium (Cd) mediated exopolysaccharide production, biofilm formation and legume root colonization ability under Cd stress to alleviate metal induced stress. RESULTS The plant was sensitive to Cd (IC 50 3–4 μg mL −1 ), whereas the bacterium showed high Cd tolerance (MIC 99 120 μg mL −1 ). Confocal laser scanning microscopy of the Cajanus cajan roots showed heavy loads of green fluorescence protein labelled Enterobacter sp. C1D on the surface of plant root, specifically at the point of root hair/lateral root formation along with cortex, even under metal stress. The root colonizing ability of Enterobacter sp. C1D was not affected by the presence of Rhizobium and the bacteria could be observed after 30 days of incubation in soil. Various plant growth parameters, antioxidant metabolites and oxidative stress indicator were significantly influenced by bacterial treatment, which, overall, reduced the adverse effect of Cd. CONCLUSION Heavy metal tolerant bacteria may be a good choice for the development of biofertilizers and may work well with the native soil microbes such as Rhizobium under the metal polluted soil. © 2019 Society of Chemical Industry