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Aqueous ethyl acetate as a novel solvent for the degreasing of black soldier fly ( Hermetia illucens L.) larvae: degreasing rate, nutritional value evaluation of the degreased meal, and thermal properties
Author(s) -
Wang Tielin,
Shen Qiao,
Feng Weiliang,
Wang Cunwen,
Yang Fang
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10131
Subject(s) - hermetia illucens , degreasing , ethyl acetate , chemistry , solvent , butyl acetate , aqueous solution , food science , meal , chromatography , biochemistry , organic chemistry , biology , larva , botany
Abstract BACKGROUND The aim of this study was to select appropriate low‐toxicity degreasing solvents to degrease black soldier fly (BSF, Hermetia illucens L.) larvae to prepare high‐quality protein. Aqueous ethyl acetate was chosen as the solvent to extract BSF protein, and traditional solvents, such as petroleum ether, n ‐hexane, and isopropanol, were chosen as controls. RESULTS The meal degreased by aqueous ethyl acetate (the volume ratio of ethyl acetate to water is 90 to 10, EA + W10) shows a high degreasing rate (29.04%), crude protein content (562.3 g kg −1 ), essential amino acid index (EAAI, 95.57), and digestible indispensable amino acid score (DIAAS, 85). The digestibility of the degreased meal samples in the simulated in vitro intestine can reach 76.52%. Thermodynamic analysis and the apparent morphology of the protein fragments showed that the meal degreased by EA + W10 exhibited thermodynamic stability, which suggests that using aqueous ethyl acetate as the degreasing solvent did not affect the nutritional value of the degreased meal. CONCLUSION The results suggest that aqueous ethyl acetate (EA + W10) can be used as a novel solvent in the degreasing of BSF larvae meal to prepare high‐quality protein with high EAAI and DIAAS and good digestibility. © 2019 Society of Chemical Industry