Premium
An erosion‐type hydrolysis behavior of insoluble protein fraction from Chlorella protothecoides
Author(s) -
Dai Laixin,
Reichert Corina L,
Hinrichs Jörg,
Weiss Jochen
Publication year - 2020
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10112
Subject(s) - hydrolysis , fraction (chemistry) , solubility , chemistry , acid hydrolysis , hydrochloric acid , chlorella , hydrolyzed protein , chromatography , biochemistry , organic chemistry , algae , biology , botany
BACKGROUND Acid‐induced hydrolysis of proteins has been used to improve the solubility and functional properties of various proteins, and could be a promising tool to facilitate the use of currently underutilized insoluble microalgae protein‐rich fractions in food applications. However, the results of a prior study showed an unusual resistance of an insoluble microalgae protein‐rich fraction to acid hydrolysis at room temperature. RESULTS In the present study, the insoluble protein‐rich fraction extracted from microalgae Chlorella prothothecoides was treated with 0.5 mol L −1 hydrochloric acid at 25, 45, 65 or 85 °C for 0–4 h. The results showed that hydrolysis of the fraction at 85 °C for 4 h led to decreases in the amount of insoluble protein‐rich aggregates and the formation of fragments with a lower molecular weight, as well as an increase in protein solubility by approximately 40%. Nevertheless, some aggregated insoluble protein‐rich particles remained, even after hydrolysis at 85 °C for 4 h. CONCLUSION The higher temperature improved the efficiency of the acid hydrolysis of the insoluble protein fraction from microalgae Chlorella prothothecoides , which is highly acid‐resistant. Overall, an erosion‐based mechanism was suggested for the acid hydrolysis of insoluble microalgae protein fraction. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.