Premium
Establishment and application of a 10‐plex liquid bead array for the simultaneous rapid detection of animal species
Author(s) -
Mei Mingzhu,
Chen Ru,
Gao Xiaobo,
Cao Yongchang,
Weng Wenchuan,
Duan Yanyu,
Tan Xin,
Liu Zhiling
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.10042
Subject(s) - donkey , animal species , mink , domestic animal , population , ingredient , biology , animal feed , food science , raw meat , veterinary medicine , zoology , medicine , ecology , environmental health
BACKGROUND Meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. To ensure the authenticity of meat products, we developed a 10‐plex xMAP assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink. RESULTS This method was investigated by analyzing DNA extracts from raw muscle, muscle mixtures, meat products and animal feeds. Our results indicated that the species of interest can be identified, differentiated and detected down to 1 g kg −1 in binary mixtures or 0.01–0.001 ng of genomic DNA from specific species. Testing of 125 commercial samples showed a 97.4% coincidence rate with the method used in routine testing in our lab. CONCLUSION These results indicated that the method established in this study could detect ten animal materials simultaneously within 3 h, which provides a new, useful tool for animal ingredient analysis in meat products and animal feeds. © 2019 Society of Chemical Industry