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Culture Medium Evaluation Using Low‐Cost Substrate for Biosurfactants Lipopeptides Production by Bacillus amyloliquefaciens in Pilot Bioreactor
Author(s) -
Lima Frederico A.,
Santos Olga Silva,
Pomella Alan William Vilela,
Ribeiro Eloízio J.,
Resende Miriam Maria
Publication year - 2020
Publication title -
journal of surfactants and detergents
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.349
H-Index - 48
eISSN - 1558-9293
pISSN - 1097-3958
DOI - 10.1002/jsde.12350
Subject(s) - bacillus amyloliquefaciens , surfactin , chemistry , bioreactor , yeast extract , fermentation , food science , central composite design , sugar , chromatography , substrate (aquarium) , response surface methodology , bacteria , bacillus subtilis , organic chemistry , oceanography , biology , genetics , geology
Abstract Total biosurfactant production by Bacillus amyloliquefaciens IT45 was evaluated with different substrates concentrations in a culture medium. A central composite design (CCD) was developed to evaluate the influence of variables, including glucose syrup, yeast extract, and calcium chloride, on surface tension (ST), total biosurfactant production, and residual sugar (RS). As a result, the best observed results for ST, RS, and total biosurfactant production were 30 mN m −1 , 31%, and 5.5 g L −1 , respectively, after 48 h of fermentations carried out in batch operation process. Characterization of the biosurfactant identified the presence of surfactin. To validate the CCD experiments, fermentations were conducted in a 40 L pilot bioreactor. For this fermentation, the cellular growth was 3.0 × 10 9 CFU mL −1 , surfactin production was 0.55 g L −1 , and RS was 28%. The results demonstrate that B. amyloliquefaciens IT45 has the potential to produce biosurfactants and does not require high concentrations of carbon and nitrogen sources for its development.