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Acid Precipitation versus Solvent Extraction: Two Techniques Leading to Different Lactone/Acidic Sophorolipid Ratios
Author(s) -
Zulkifli Wan Nur Fatihah Wan Muhammad,
Razak Nurul Nadhirah Abdul,
Yatim Abdul Rashid M.,
Hayes Douglas G.
Publication year - 2019
Publication title -
journal of surfactants and detergents
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.349
H-Index - 48
eISSN - 1558-9293
pISSN - 1097-3958
DOI - 10.1002/jsde.12223
Subject(s) - chemistry , chromatography , extraction (chemistry) , solvent , lactone , electrospray ionization , mass spectrometry , electrospray , precipitation , high performance liquid chromatography , protein precipitation , organic chemistry , physics , meteorology
Sophorolipids (SL) prepared from biotransformation of refined bleached deodorized (RBD) palm olein by Starmerella bombicola (ATCC 22214) occur as a mixture of homologs. Characterization of SL was performed using high‐performance liquid chromatography with electrospray ionization mass spectrometry detection (HPLC‐ESI‐MS) under negative ion mode. Two different purification methods were compared: precipitation and solvent extraction. SL purified by precipitation and solvent extraction consisted of five major homologs with m/z values of 687, 645, 689, 705, and 661. The fractions of lactone and acidic SL via precipitation were 70.24 ± 1.4% and 29.76 ± 1.4%, respectively. SL isolated by solvent extraction contained 44.78 ± 1.9% lactone and 55.22 ± 1.9% acidic SL. Therefore, the purification method strongly impacted the composition of the SL products.