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Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy
Author(s) -
Huang YuSan,
Karashima Takeshi,
Yamamoto Masayuki,
Hamaguchi Hiroo
Publication year - 2003
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.960
Subject(s) - raman spectroscopy , yeast , confocal , mitosis , chemistry , resolution (logic) , nucleus , biophysics , analytical chemistry (journal) , nuclear magnetic resonance , optics , biology , physics , biochemistry , microbiology and biotechnology , chromatography , artificial intelligence , computer science
The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time‐ and space‐resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi‐channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time‐dependent changes of major molecular components during the cell cycle were elucidated. Copyright © 2002 John Wiley & Sons, Ltd.