Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy | Zendy

Huang YuSan | Zendy; Karashima Takeshi | Zendy; Yamamoto Masayuki | Zendy; Hamaguchi Hiroo | Zendy

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Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy

Author(s) -

Huang YuSan,

Karashima Takeshi,

Yamamoto Masayuki,

Hamaguchi Hiroo

Publication year - 2003

Publication title -

journal of raman spectroscopy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.748

H-Index - 110

eISSN - 1097-4555

pISSN - 0377-0486

DOI - 10.1002/jrs.960

Subject(s) - raman spectroscopy , yeast , confocal , mitosis , chemistry , resolution (logic) , nucleus , biophysics , analytical chemistry (journal) , nuclear magnetic resonance , optics , biology , physics , biochemistry , microbiology and biotechnology , chromatography , artificial intelligence , computer science

The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time‐ and space‐resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi‐channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time‐dependent changes of major molecular components during the cell cycle were elucidated. Copyright © 2002 John Wiley & Sons, Ltd.

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