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Comparison of conventional and shifted excitation Raman difference spectroscopy for bacterial identification
Author(s) -
Lorenz Björn,
Guo Shuxia,
Raab Christoph,
Leisching Patrick,
Bocklitz Thomas,
Rösch Petra,
Popp Jürgen
Publication year - 2022
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.6360
Subject(s) - raman spectroscopy , spectroscopy , analytical chemistry (journal) , biomolecule , chemistry , fluorescence , materials science , optics , chromatography , biochemistry , physics , quantum mechanics
Raman spectroscopy is an emerging tool for fast bacterial identification. However, Raman spectroscopy is depending on suitable preprocessing of the spectra, thereby background removal is a decisive step for conventional Raman spectroscopy. The background has to be estimated, which is challenging especially for high fluorescence backgrounds. Shifted excitation Raman difference spectroscopy (SERDS) eliminates the background through the experimental procedure and holds as promising approach for highly fluorescent samples. Bacterial Raman spectra might be especially complex because these spectra consist of a multitude of overlapping Raman bands from a large multiplicity of biomolecules and only subtitle differences between the species Raman spectra enable the bacterial identification. Here, we investigate the benefits of SERDS compared with conventional Raman spectroscopy specific for the study and identification of bacteria. The comparison utilizes spectra sets of four bacterial species measured with conventional Raman spectroscopy and SERDS and covers three processing approaches for SERDS spectra, for example, the reconstruction with a non‐negative least square algorithm.