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Single‐beam dual‐color alternate‐pathway two‐photon spectroscopy: Toward an optical toolbox for redox biology
Author(s) -
Chebotarev Artem S.,
Lanin Aleksandr A.,
Raevskii Roman I.,
Kostyuk Alexander I.,
Smolyarova Daria D.,
Bilan Dmitry S.,
Savitskii Ilya V.,
Fedotov Andrei B.,
Belousov Vsevolod V.,
Zheltikov Aleksei M.
Publication year - 2021
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.6183
Subject(s) - spectroscopy , laser , optics , photonics , optoelectronics , materials science , absorption (acoustics) , fluorescence , photon , physics , quantum mechanics
We demonstrate a versatile single‐laser platform for single‐beam dual‐color two‐photon spectroscopy that combines a short‐pulse laser source with a tunable broadband wavelength converter based on a highly nonlinear photonic‐crystal fiber (PCF). We show that the short‐pulse PCF output can be tailored, via dispersion and nonlinearity management, to deliver a broadband optical driver whose spectral structure is ideally suited for a single‐beam two‐photon absorption (2PA) spectroscopy of the next‐generation genetically encodable fluorescent‐protein (FP) sensors of pH and redox‐responsive contrast agents. As a promising spectroscopic resource for redox biology, the short‐pulse PCF output can be finely sculpted to alternately drive an FP system via a one of its two 2PA‐allowed quantum pathways, yielding a high‐contrast fluorescence readout for a highly sensitive detection of redox reactions and signaling, pH sensing, and oxidative‐stress diagnosis.