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Detection and quantification of small concentrations of moxifloxacin using surface‐enhanced Raman spectroscopy in a Kretschmann configuration
Author(s) -
Mohaghegh Faezeh,
Mazaheri Tehrani Alireza,
Rana Debkumar,
Winterhalter Mathias,
Materny Arnulf
Publication year - 2021
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.6116
Subject(s) - raman spectroscopy , analyte , chemistry , surface enhanced raman spectroscopy , substrate (aquarium) , analytical chemistry (journal) , metal , laser , molecule , optics , raman scattering , chromatography , physics , oceanography , organic chemistry , geology
A relatively simple experimental procedure is proposed for the simultaneous detection and quantitative assessment of moxifloxacin (Moxi) as an example for an antibiotic using surface‐enhanced Raman spectroscopy (SERS) performed in a Kretschmann configuration (KC). The example of Moxi shows the advantage of this approach, such as high sensitivity and relatively simple experimental procedure. The Moxi was reliably detected at levels of 100 nM using excitation laser powers as low as a few milliwatts. We also demonstrate that in the KC, the direct coupling between the electronic systems of analyte molecules and metal substrate contributing to the chemical enhancement mechanism in SERS plays a major role. For this, we have performed simulations based on density functional theory (DFT). The line profile of the SERS spectra can be explained by the direct coupling of molecular sites to the metal. This adds to the molecular specificity of SERS when using the KC.