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532‐nm‐excited hyper‐Raman spectroscopy of amino acids
Author(s) -
Wen ChunI,
Yu ChiNan,
Thirumalaivasan Natesan,
Hiramatsu Hirotsugu
Publication year - 2021
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.6039
Subject(s) - raman spectroscopy , amino acid , spectral line , chemistry , excited state , excitation wavelength , excitation , intensity (physics) , spectroscopy , aqueous solution , wavelength , analytical chemistry (journal) , materials science , optics , biochemistry , chromatography , atomic physics , physics , optoelectronics , quantum mechanics , astronomy
This paper reports the hyper‐Raman (HR) spectra of the 20 amino acids in aqueous solution in the range of 400–1,800 cm −1 with an excitation wavelength of 532 nm. A remarkable common feature in the nonresonance HR spectra is the large intensity of the HR bands of the COO − group. Whereas the peak position is mostly identical between the HR and Raman spectra, the intensity pattern is not. We discuss the similarities and dissimilarities between the pattern of the two spectra of each amino acid and give possible assignments to each HR band by comparing them with those of the corresponding Raman band. This study offers a reference for the HR spectra of the amino acids as the basic building block of proteins. It helps interpret the HR spectra of proteins and peptides.