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High‐speed broadband Fourier‐transform coherent anti‐stokes Raman scattering spectral microscopy
Author(s) -
Kinegawa Ryo,
Hiramatsu Kotaro,
Hashimoto Kazuki,
Badarla Venkata Ramaiah,
Ideguchi Takuro,
Goda Keisuke
Publication year - 2019
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.5630
Subject(s) - optics , microscopy , microscope , spectral imaging , dwell time , raman spectroscopy , materials science , fourier transform , raman scattering , broadband , optical microscope , pixel , spectral resolution , frame rate , resolution (logic) , physics , scanning electron microscope , computer science , spectral line , artificial intelligence , medicine , clinical psychology , quantum mechanics , astronomy
We demonstrate broadband Fourier‐transform coherent anti‐Stokes Raman scattering (FT‐CARS) spectral microscopy with a pixel dwell time of 42 μs, which is ~50 times shorter than the shortest‐to‐date pixel dwell time for CARS spectral microscopy. Our broadband FT‐CARS spectral microscope is composed of an FT‐CARS spectrometer, a rapid galvanometric scanner, and a high‐speed image acquisition circuit, enabling a frame rate of 2.4 fps with a pixel resolution of 100 × 100 pixels, a bandwidth of 600–1,200 cm −1 , a spatial resolution of 0.95 μm, and a spectral resolution of 37 cm −1 . As a proof‐of‐principle demonstration, we used the high‐speed FT‐CARS spectral microscope to perform CARS imaging of polymer beads and Haematococcus lacustris cells. Our high‐speed broadband CARS spectral microscope holds promise for studying rapid cellular dynamics, such as signaling, cell‐to‐cell communication, and molecular transport in a label‐free manner.