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Rapid and sensitive surface‐enhanced resonance Raman spectroscopy detection for norepinephrine in biofluids
Author(s) -
Zhou Xia,
Qin Miao,
Zhu Jun,
Wang Cong,
Zhu Guang,
Wang Hongyan,
Yang Liangbao
Publication year - 2019
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.5519
Subject(s) - raman spectroscopy , chemistry , analyte , resonance raman spectroscopy , ferric , analytical chemistry (journal) , chromophore , nanoparticle , surface enhanced raman spectroscopy , biosensor , resonance (particle physics) , detection limit , nanotechnology , chromatography , inorganic chemistry , photochemistry , materials science , raman scattering , biochemistry , physics , particle physics , optics
Norepinephrine (NE) is a kind of catecholamine neurotransmitter that participates significant function in living organism. Rapid and sensitive detection target in complex system is crucial in analytical science. A facile ferric citrate (CA‐Fe (III)) sensor was designed successfully for sensitive detection of NE by surface‐enhanced resonance Raman spectroscopy (SERRS) method. This CA‐Fe (III) sensor was prepared by modifying Fe (III) on the surface of uniform Au nanoparticles (NPs) wrapped with citrate (CA) stabilizer. The implementation of NE selective detection is attributed to significant enhancement provided by Au NPs and additional enhancement provided by ligand‐to‐metal charge transfer between chromophore and analyte. In addition, a universal CH 3 OH, HCl, and petroleum ether added in sequence pretreatment method was developed and applied to purify and enrich the target in practical biological sample, realizing selective detection of NE in biofluids. This novel sensor combined with SERRS and pretreatment strategy not only amplifies the Raman signal of NE but also paves the way for pretreating sample in complex system.