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A built‐in surface‐enhanced Raman scattering‐active microneedle for sampling in vivo and surface‐enhanced Raman scattering detection ex vivo of NO
Author(s) -
Han Shuyan,
Sun Jie,
Wang Jieyu,
Qian Weiping,
Dong Jian
Publication year - 2018
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.5469
Subject(s) - ex vivo , raman scattering , materials science , in vivo , nanotechnology , raman spectroscopy , chemistry , optics , physics , microbiology and biotechnology , biology
For sampling in vivo and surface‐enhanced Raman scattering (SERS) detection ex vivo, a built‐in SERS‐active microneedle is fabricated by adsorbing gold nanoshells (GNSs; as built‐in SERS‐active materials) in a corroded groove on an acupuncture needle. 3,4‐diaminobenzene‐thiol adsorbed on the GNSs is used as a selective SERS probe to detect nitric oxide (NO). The SERS‐active microneedles take SERS probes into the tissues of living mice with minimal invasion to react with NO selectively and then they are pulled out for direct and rapid SERS detection without any pretreatment steps or tedious operations. The built‐in SERS‐active microneedle would become a versatile platform for sampling in vivo and SERS detection ex vivo, taking SERS beyond culture cells to living animals.