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Raman spectroscopy as a novel method in placental research: Recognizing the pattern of placental hypoxia
Author(s) -
SchlabritzLoutsevitch Natalia,
Gandhi Kushal,
Soydemir Fatimah,
Brownbill Paul,
Sengar Raghvendra,
Ventolini Gary,
Bruillard Paul,
Gosink Luke
Publication year - 2017
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.5229
Subject(s) - raman spectroscopy , perfusion , hypoxia (environmental) , placenta , ex vivo , chemistry , fetus , nuclear magnetic resonance , biophysics , biology , medicine , oxygen , biochemistry , physics , optics , in vitro , genetics , pregnancy , organic chemistry
Raman spectroscopy (RS) is a nonelastic photon scattering technique. We evaluated whether a specific RS pattern exists in fetal venous perfusates obtained at 30‐ to 60‐min intervals from the ex vivo human dual placental perfusion model under hypoxic ( n = 6) and normoxic ( n = 5) conditions. A stratified principle component and a linear support vector machine analyses showed that the separation between 2 conditions was readily feasible at 60–120 min of perfusion; after this, any apparent differences were most likely the result of artifact overfitting. This report is the first attempt to identify the RS fingerprint associated with placental hypoxia.