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Rapid quantification of total protein with surface‐enhanced Raman spectroscopy using o ‐phthalaldehyde
Author(s) -
Eryilmaz Merve,
Zengin Adem,
Boyaci Ismail Hakki,
Tamer Uğur
Publication year - 2017
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.5099
Subject(s) - raman spectroscopy , o phthalaldehyde , bovine serum albumin , calibration curve , detection limit , chemistry , surface enhanced raman spectroscopy , analytical chemistry (journal) , colloidal gold , ultraviolet , chromatography , linear range , nanoparticle , materials science , raman scattering , nanotechnology , derivatization , mass spectrometry , optics , optoelectronics , physics
In this study, surface‐enhanced Raman spectroscopy (SERS) based quantification method for the total protein using o ‐phthalaldehyde is reported for the first time. For this purpose, o ‐phthalaldehyde was chosen to form a complex with protein, and SERS signal was observed in the presence of gold nanoparticles. A calibration curve was obtained by plotting the intensity of the SERS signal at 727 cm −1 versus the concentration of protein standard (bovine serum albumin, BSA). Thus, the correlation was found to be linear within the range of 0.054–0.72 mg/ml of BSA, and the limit of detection was determined to be 0.08 mg/ml. All tests were carried out using a portable Raman instrument with an analysis time of 5 min. In addition, the ability of the developed method to quantify total protein in milk samples was investigated, and the obtained results were compared to the conventional ultraviolet methods. Copyright © 2017 John Wiley & Sons, Ltd.