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Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper‐spectral coherent anti‐Stokes Raman microscopy using a sub‐nanosecond supercontinuum laser source
Author(s) -
Ishitsuka Kei,
Koide Masahiro,
Yoshida Masaki,
Segawa Hiroki,
Leproux Philippe,
Couderc Vincent,
Watanabe Makoto M.,
Kano Hideaki
Publication year - 2017
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4979
Subject(s) - raman spectroscopy , squalene , intracellular , lipid droplet , supercontinuum , chemistry , raman scattering , microscopy , biophysics , photochemistry , analytical chemistry (journal) , biochemistry , optics , biology , physics , chromatography , organic chemistry , photonic crystal fiber , fiber
We applied hyper‐spectral coherent anti‐Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W‐13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis CC stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy. Copyright © 2016 John Wiley & Sons, Ltd.