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Hyperspectral coherent Raman imaging – principle, theory, instrumentation, and applications to life sciences
Author(s) -
Kano Hideaki,
Segawa Hiroki,
Okuno Masanari,
Leproux Philippe,
Couderc Vincent
Publication year - 2016
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4853
Subject(s) - hyperspectral imaging , raman scattering , raman spectroscopy , chemical imaging , coherent anti stokes raman spectroscopy , microscopy , optics , fluorescence lifetime imaging microscopy , fluorescence , x ray raman scattering , signal (programming language) , materials science , nanotechnology , physics , chemistry , computer science , artificial intelligence , programming language
Coherent Raman scattering microscopy such as coherent anti‐Stokes Raman scattering and stimulated Raman scattering microscopy boosts the weak Raman signal and enables us to perform label‐free visualization of the molecular distribution and its dynamical behavior in living cells and tissues with high speed. In comparison with fluorescence imaging, cells and tissues can be visualized without specifying the target molecule. In this review, we describe the characteristics of a hyperspectral coherent Raman imaging method, which is capable of acquiring both spectra and images simultaneously, and review applications to life sciences. Copyright © 2015 John Wiley & Sons, Ltd.