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Detection of total protein in milk using phosphomolybdic acid‐mediated surface‐enhanced Raman spectroscopy
Author(s) -
Huang Yingzhao,
Gu Jianming,
Xiang Gang,
Xu Jiajie,
Fu Shuilin,
Gong Heng
Publication year - 2016
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4812
Subject(s) - phosphomolybdic acid , chemistry , detection limit , raman spectroscopy , casein , chromatography , bovine serum albumin , bovine milk , raman scattering , analytical chemistry (journal) , biochemistry , physics , optics , catalysis
Although many surface‐enhanced Raman scattering (SERS)‐based methods for detecting specific proteins have been studied, simple and direct detection of total protein in liquid using a SERS‐based method remains difficult. In this study, a distinguishable effect on the SERS spectra from pre‐mixture of phosphomolybdic acid (PMA) with protein was found, indicating that PMA could be used as a SERS reporter for total protein detection in a liquid sample. Further experiments confirmed a good linear relationship between a premixed concentration of protein (casein, whey protein or bovine serum albumin) and the SERS intensity of PMA in our SERS system. Using casein as a reference, a PMA‐mediated SERS method was proposed that can quantitatively analyze protein at 2.5–25 µg/ml with a limit of detection of 1.5 µg/ml. Our PMA‐mediated SERS method is a simple and rapid method for quantitative analysis of total protein in milk. Copyright © 2015 John Wiley & Sons, Ltd.