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Preparation of SERS active Ag nanoparticles encapsulated by phospholipids
Author(s) -
An Hyeun Hwan,
Han Won Bae,
Kim Yongdeok,
Kim HeeSoo,
Oh Yoong,
Yoon Chong Seung
Publication year - 2014
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4461
Subject(s) - rhodamine 6g , raman spectroscopy , nanoparticle , materials science , membrane , nanotechnology , surface enhanced raman spectroscopy , chemical engineering , lipid bilayer , chemistry , molecule , raman scattering , organic chemistry , biochemistry , physics , engineering , optics
A cost‐effective way of fabricating lipid‐coated surface‐enhanced Raman spectroscopy (SERS) substrate having reproducible high SERS activity was proposed. Ag nanoparticle embedded in 1,2‐dioleoyl‐ sn ‐glycero‐3‐phosphocholine (DOPC) and 1,2‐dioleoyl‐3‐trimethylammonium‐propane (DOTAP) membranes was produced by direct deposition of a 5‐nm‐thick layer of Ag onto the solid‐supported phospholipid membrane, and subsequent dissolution of the Ag nanoparticle‐embedded membrane in iso‐octane allowed easy one‐pot fabrication of DOPC‐ or DOTAP‐coated Ag nanoparticles. In particular, DOTAP produced nearly monodisperse lipid‐encapsulated Ag nanoparticles (9 nm in diameter) exhibiting reproducible high SERS activity (detecting up to 10 nM of rhodamine 6G and 0.5 μM of glutathione). In addition, the process was modified to incorporate variety of Raman active molecules (rhodamine 6G, malachite green, 4‐aminothiopheonol, 4‐mercaptopyridine) into the particle‐encapsulating lipid bilayer. The DOTAP/Raman dye‐coated Ag nanoparticles also generated high SERS activity to enable potential application of the DOTAP/Raman dye‐coated Ag nanoparticles feasible in different areas. Copyright © 2014 John Wiley & Sons, Ltd.