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A SERS‐active enzymatic product used for the quantification of disease‐related molecules
Author(s) -
Yu Zhi,
Chen Lei,
Wang Yue,
Wang Xu,
Song Wei,
Ruan Weidong,
Zhao Bing,
Cong Qian
Publication year - 2014
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4425
Subject(s) - bioanalysis , chromogenic , detection limit , chemistry , horseradish peroxidase , combinatorial chemistry , raman scattering , catalysis , raman spectroscopy , enzyme , linear range , nanotechnology , chromatography , materials science , biochemistry , optics , physics
In this paper, a method for enhancing the analysis of biologic materials was designed. This method incorporated the surface‐enhanced Raman scattering (SERS) technique and an enzyme catalysis‐based bioassay to develop a more efficient analysis procedure. Using this combination, the quick and simple detection of disease‐related molecules (the human cardiac isoform of troponin T, cTnT) in human serum was achieved. This method utilizes enzyme catalysis to develop a H 2 O 2 ‐horseradish peroxidase (HRP)‐3,3′,5,5′‐tetramethylbenzidine (TMB) chromogenic system, and the final enzymatic product TMB 2+ revealed perfect SERS activities. By analyzing a concentration‐dependent SERS spectrum, the quantitative analysis was accomplished. Our findings reveal that the proposed method has a wider linear range and a more sensitive detection limit compared with traditional chromogenic tests. In summary, two existing methods have been combined to create a new model, which has the potential to be used for the bioanalysis and early diagnosis of diseases. Copyright © 2013 John Wiley & Sons, Ltd.