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Role of Phe‐D 5 isotopically labeled analogues of bradykinin on elucidation of its adsorption mode on Ag, Au, and Cu electrodes. Surface‐enhanced Raman spectroscopy studies
Author(s) -
Proniewicz Edyta,
Ignatjev Ilja,
Niaura Gediminas,
Sobolewski Dariusz,
Prahl Adam,
Proniewicz Leonard M.
Publication year - 2013
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4343
Subject(s) - chemistry , raman spectroscopy , adsorption , electrochemistry , electrode , aqueous solution , phenylalanine , deuterium , raman scattering , bradykinin , stereochemistry , analytical chemistry (journal) , nuclear chemistry , inorganic chemistry , amino acid , organic chemistry , biochemistry , physics , receptor , quantum mechanics , optics
Raman (RS), surface‐enhanced Raman scattering (SERS), electrochemistry, and isotopic effect methods were used to characterize selective adsorption of two isotopically labeled bradykinin analogues, [(Phe‐D 5 ) 5 ]BK and [(Phe‐D 5 ) 8 ]BK, a hormone which is known to be involved in small‐cell and non‐small‐cell lung carcinoma and prostate cancer. The investigated analogues contain Phe residue, at position 5 or 8 in the amino acid sequence, substituted by Phe‐D 5 (five protons of L‐phenylalanine ring substituted by deuterium). [(Phe‐D 5 ) 5 ]BK and [(Phe‐D 5 ) 8 ]BK were immobilized onto electrochemically roughened Ag, Au, and Cu electrode surfaces under different applied electrode potentials (−1.000 V to 0.200 V) in an aqueous solution containing 0.01 M phosphate buffer (pH = 7.0) and 0.1 M Na 2 SO 4 . Based on the analyses of the spectral profiles in the 920 – 1050 cm −1 spectral range, specific conclusions were drawn with respect to the Phe⋅⋅⋅metal interactions and changes in the interaction that occurred when the adsorption conditions were varied. Copyright © 2013 John Wiley & Sons, Ltd.