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Multimodal, label‐free nonlinear optical imaging for applications in biology and biomedical science
Author(s) -
Mouras R.,
Bagnaninchi P.,
Downes A.,
Elfick A.
Publication year - 2013
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4305
Subject(s) - ex vivo , regenerative medicine , microscopy , in vivo , optical imaging , nanotechnology , biomedical engineering , preclinical imaging , drug delivery , nonlinear optical , molecular imaging , stem cell , cancer cell , computer science , cancer , materials science , biophysics , biology , microbiology and biotechnology , medicine , pathology , nonlinear system , physics , optics , quantum mechanics , genetics
We have developed a multimodal optical platform for label‐free imaging on the basis of nonlinear optical microscopy (NLO) for applications in biology and biomedical science. Three application areas have been chosen as examples: regenerative medicine, drug delivery monitoring and cancer diagnosis. Obtained data showed the potential of NLO microscopy for the following: (1) investigating the stem cell differentiation states prior their use in regenerative medicine and tissue repair, (2) tracking drug molecules in living cells and (3) detecting cancer by imaging biopsies ex vivo . The results of this study illustrate the potential to translate NLO microscopy from ex vivo to in vivo applications. Copyright © 2013 John Wiley & Sons, Ltd.