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Investigation of organelle‐specific intracellular water structures with Raman microspectroscopy
Author(s) -
Tiwari Shraeddha,
Ando Masahiro,
Hamaguchi Hiroo
Publication year - 2013
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4165
Subject(s) - biomolecule , raman spectroscopy , organelle , raman microspectroscopy , intracellular , molecule , chemistry , hydrogen bond , deuterium , biophysics , chemical physics , nanotechnology , materials science , biochemistry , biology , physics , organic chemistry , optics , quantum mechanics
The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle‐specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen‐bonding network of water molecules. Copyright © 2012 John Wiley & Sons, Ltd.