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Multicomponent Rayleigh scattering from guanidine hydrochloride solutions
Author(s) -
Svanidze Anna V.,
Lushnikov Sergey G.,
Romanov Vadim P.,
Kojima Seiji
Publication year - 2012
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.4059
Subject(s) - guanidine , brillouin scattering , rayleigh scattering , hydrochloride , chemistry , debye , relaxation (psychology) , scattering , spectral line , brillouin zone , molecular physics , nuclear magnetic resonance , analytical chemistry (journal) , condensed matter physics , optics , physics , chromatography , organic chemistry , quantum mechanics , psychology , social psychology , optical fiber
We present results of Brillouin light scattering studies of lysozyme and guanidine hydrochloride solutions in the temperature range 290–350 K. The Brillouin spectra of 6 M guanidine hydrochloride have been found to contain an additional component in Rayleigh scattering that manifests itself as a broad quasi‐elastic scattering line centered at the unshifted frequency and described by a Lorentz function (i.e. a Debye relaxor with relaxation time τ 1 ~ 25 ps at room temperature). The temperature dependence of τ 1 is described by the Arrhenius law with activation energy E a = 0.11 ± 0.01 eV and prefactor τ 0 = 0.33 ± 0.03 ps. The Brillouin spectra of lysozyme denatured by 6 M guanidine hydrochloride exhibit a more complicated structure of the additional contribution into Rayleigh scattering, which is fitted best of all by a sum of two Lorentzians centered at the unshifted frequency (with relaxation times τ 1 ~ 19 ps and τ 2 ~ 180 ps at 339 K). Possible origins of the quasi‐elastic scattering are discussed. Copyright © 2012 John Wiley & Sons, Ltd.