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Quantitative and multicomponent analysis of prevalent urinary calculi using Raman spectroscopy
Author(s) -
Chiu Yichun,
Huang YiYu,
Chen PoAn,
Lu ShingHwa,
Chiu Allen W.,
Chiang Huihua Kenny
Publication year - 2012
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.3138
Subject(s) - chemistry , ternary operation , raman spectroscopy , uric acid , oxalate , detection limit , calcium oxalate , quantitative analysis (chemistry) , chromatography , nuclear chemistry , analytical chemistry (journal) , inorganic chemistry , biochemistry , physics , optics , computer science , programming language
This study established a quantitative micro‐Raman spectroscopic (MRS) method for measuring multicomponents (binary and ternary compositions) of prevalent urine calculi extracted from the ureter after the ureteroscopic lithotripsy (URSL) procedure. The analysis used calibration curves of known mixtures of synthetically prepared calcium oxalate monohydrate (COM), hydroxyapatite (HAP), calcium oxalate dehydrate (COD), dicalcium phosphate dehydrate (DCPD), and uric acid. A variety of samples of binary and ternary mixtures including COM/HAP, COM/COD, COD/HAP, COM/uric acid, COD/uric acid, HAP/uric acid, HAP/DCPD, and COM/COD/HAP were prepared in various concentration ratios for use as the basis of the quantitative analysis. Intensities of the characteristic bands at 961 cm −1 ( I HAP ), 986 cm −1 ( I DCPD ), 1402 cm −1 ( I UricAcid ), 1462 cm −1 ( I COM ), and 1477 cm −1 ( I COD ) were used for the calculation. We derived a set of quantitative analysis equations for the ternary composition COD/COM/HAP group by combining two binary equations from the groups COM/COD and the HAP/COM. This study quantitatively measured 18 urine samples extracted from the 18 patients' ureters after the URSL procedure. Fifteen samples were binary mixtures, whereas three samples were ternary mixtures. This research successfully applied the quantitative MRS‐based analysis technique from bench to bedside. Copyright © 2012 John Wiley & Sons, Ltd.