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Quantification of purine basis in their mixtures at femto‐molar concentration levels using FT‐SERS
Author(s) -
Ranc Vaclav,
Hruzikova Jana,
Maitner Kamil,
Prucek Robert,
Milde David,
Kvítek Libor
Publication year - 2012
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.3124
Subject(s) - raman scattering , hypoxanthine , raman spectroscopy , chemistry , xanthine , molar ratio , purine , nanoscopic scale , analytical chemistry (journal) , nanoparticle , nanotechnology , materials science , chromatography , optics , organic chemistry , enzyme , catalysis , physics
Surface‐enhanced Raman scattering spectroscopy represents one of the unique techniques for studying nanoscale objects, and its distinctive properties can be used in the process of further analysis. The careful evaluation of the particular influence of selected key‐role experimental parameters (e.g. pH value of measured sample mixture, size and distribution of used nanoparticles) and the influence of reduction agent used in the process of formation of desired nanoparticle objects presents an important task in the further study of surface‐enhanced Raman scattering effect. A broad study of these experimental parameters was performed in this paper. The main aim of the presented work was to a demonstrate an application potential of selected experimental conditions in the determination of three purine bases: adenine, xanthine, and hypoxanthine. The resulting limits of detection are at femtomolar concentration levels for all three studied compounds. Copyright © 2011 John Wiley & Sons, Ltd.