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SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide–antibody assays
Author(s) -
Virga Alessandro,
Rivolo Paola,
Descrovi Emiliano,
Chiolerio Alessandro,
Digregorio Gabriella,
Frascella Francesca,
Soster Marco,
Bussolino Federico,
Marchiò Serena,
Geobaldo Francesco,
Giorgis Fabrizio
Publication year - 2012
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.3086
Subject(s) - raman spectroscopy , raman scattering , nanoparticle , plasmon , porous silicon , surface plasmon resonance , chemistry , molecule , materials science , analytical chemistry (journal) , nanomaterials , silicon , nanotechnology , photochemistry , optoelectronics , organic chemistry , optics , physics
Ag nanoparticles synthesized on porous silicon samples were studied and applied as substrates for surface‐enhanced Raman scattering (SERS). The metallic nanostructures prepared by immersion plating were characterized by UV–Vis reflectance spectroscopy and scanning electron microscopy. SERS activity of the substrates was tested using Cyanine dye 1,3,3,1′,3′,3′‐esamethyl‐5,5′‐dimethoxyindodicarbocyanine iodide (Cy5‐OCH 3 ) as a probe molecule. The Raman spectra obtained for different excitation wavelengths indicate amplifications ascribed to plasmonic resonances with an enhancement factor up to 10 7 . CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays. Copyright © 2011 John Wiley & Sons, Ltd.