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Optical sectioning using single‐plane‐illumination Raman imaging
Author(s) -
Barman Ishan,
Tan Khay Ming,
Singh Gajendra Pratap
Publication year - 2010
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.2785
Subject(s) - raman spectroscopy , optical sectioning , confocal , microscopy , optics , materials science , resolution (logic) , confocal microscopy , light sheet fluorescence microscopy , raman microscope , superresolution , raman scattering , computer science , physics , scanning confocal electron microscopy , artificial intelligence , image (mathematics)
Abstract Confocal Raman imaging is widely used for optical sectioning of materials. However, for biological applications it often suffers from poor axial resolution, photodamage to the sample of interest, substrate interference, and long acquisition times. We have applied the principles of light sheet microscopy to Raman imaging and show for the first time that optically sectioned Raman images can be obtained in significantly lower acquisition times. Copyright © 2010 John Wiley & Sons, Ltd.