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Fast three‐dimensional chemical imaging by interferometric multiplex coherent anti‐Stokes Raman scattering microscopy
Author(s) -
Sung Jiha,
Chen BiChang,
Lim SangHyun
Publication year - 2011
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.2647
Subject(s) - hyperspectral imaging , microscopy , optics , raman spectroscopy , sensitivity (control systems) , raman scattering , chemical imaging , signal (programming language) , interferometry , fourier transform , apodization , chemistry , materials science , analytical chemistry (journal) , physics , artificial intelligence , chromatography , quantum mechanics , programming language , engineering , electronic engineering , computer science
We report significant improvements in both signal sensitivity and imaging speed of Fourier transform spectral interferometry coherent anti‐Stokes Raman scattering (FTSI‐CARS) microscopy. With a help of an apodization function in the signal retrieval process, background due to the spectral change of nonresonant signals is eliminated. We experimentally verify that the sensitivity of the improved method is nearly shot‐noise‐limited. The current maximum detection sensitivity is ∼10 mM of aqueous sulfate ions, which correspond to ∼10 6 oscillators in the microscopy focal volume. Operating the charge‐coupled device (CCD) in the crop mode increases the image acquisition speed by more than ten times. A vibrational hyperspectral image of a polymer sample with 100 × 100 pixel can be obtained within 3 s. With the improved sensitivity and speed, we also perform three‐dimensional volume imaging. Superior chemical selectivity is demonstrated with a mixture of two different oil droplets, which have identical vibrational peak positions but different relative peak ratios. Copyright © 2010 John Wiley & Sons, Ltd.