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Use of peptide for selective and sensitive detection of an Anthrax biomarker via peptide recognition and surface‐enhanced Raman scattering
Author(s) -
Ryu Kyungtag,
Haes Amanda J.,
Park HyeYeon,
Nah Sanghee,
Kim Joungmok,
Chung Hoeil,
Yoon MoonYoung,
Han SungHwan
Publication year - 2010
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.2591
Subject(s) - peptide , conjugated system , chemistry , antibody , biomarker , raman scattering , peptide sequence , raman spectroscopy , combinatorial chemistry , microbiology and biotechnology , biochemistry , biology , immunology , gene , organic chemistry , optics , physics , polymer
A short 16‐amino acid peptide has been used in place of an antibody to selectively detect the specific Anthrax biomarker, protective antigen (PA), using surface‐enhanced Raman scattering (SERS). Peptides are more stable than antibodies under various biological conditions and are easily synthesized for a specific target. A peptide that has high affinity to PA was conjugated onto gold nanoparticles along with a Raman reporter and then incubated in various concentrations of PA. Parallel studies in which the peptide sequence was replaced with an antibody were performed to compare the performance of the two methodologies. Both the peptide and antibody functionalized nanoparticles were able to specifically detect PA concentrations down to 6.1 f M . These results demonstrate that these short, robust peptides can be used in the place of traditional antibodies to specifically recognize target biomarkers in the field for the potential diagnosis of disease. Copyright © 2010 John Wiley & Sons, Ltd.