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Detection of biologically active diterpenoic acids by Raman Spectroscopy
Author(s) -
Talian Ivan,
Oriňák Andrej,
Efremov Evtim V.,
Ariese Freek,
Kaniansky Dušan,
Oriňáková Renáta,
Hübner Jörg
Publication year - 2010
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.2545
Subject(s) - raman spectroscopy , chemistry , analytical chemistry (journal) , spectroscopy , resonance raman spectroscopy , polarizability , substrate (aquarium) , molecule , chromatography , organic chemistry , optics , physics , oceanography , quantum mechanics , geology
Three poorly detectable, biologically active diterpenoic acids, kaurenoic, abietic, and gibberellic acid, were studied by using different modes of Raman spectroscopy. Because of their structural similarities, in the absence of strongly polarizable groups, conventional Raman spectroscopy is not suitable for their unambiguous identification, especially not in solution. We attempted to increase the sensitivity by applying UV‐resonance Raman spectroscopy and surface‐enhanced Raman spectroscopy (SERS) techniques. The UV‐Raman spectra of the three compounds in ethanol/water 50:50 showed only very few enhanced Raman lines. SERS spectra with 514‐nm excitation with Ag colloids were also relatively weak. The best SERS spectra were obtained with 785‐nm excitation on a novel nanostructured substrate, ‘black silicon’ coated with a 400‐nm gold layer. The spectra showed clear differences, and these ‘fingerprints’ would be suitable for the unambiguous identification of these diterpenoic acids. Copyright © 2009 John Wiley & Sons, Ltd.