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Different contrast information obtained from CARS and nonresonant FWM images
Author(s) -
Akimov Denis,
Chatzipapadopoulos Susana,
Meyer Tobias,
Tarcea Nicolae,
Dietzek Benjamin,
Schmitt Michael,
Popp Jürgen
Publication year - 2009
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.2205
Subject(s) - contrast (vision) , interference (communication) , optics , raman scattering , refractive index , refraction , mixing (physics) , signal (programming language) , raman spectroscopy , physics , four wave mixing , scattering , microscopy , absorption (acoustics) , topology (electrical circuits) , chemistry , nonlinear optics , computer science , mathematics , telecommunications , quantum mechanics , laser , channel (broadcasting) , combinatorics , programming language
We demonstrate that coherent anti‐Stokes Raman scattering (CARS) microscopy can be used not to obtain vibrational contrast related to a particular chemical species only, but furthermore, it directly yields topological contrast. Topological contrast is due to the spatially dependent refractive index of the sample and a number of linear effects as interference, refraction and absorption contribute to the topological contrast inherent to CARS images. In order to distinguish between different contrast mechanisms we propose an image correction method based on the analysis of the nonresonant four‐wave mixing (FWM) signal distribution. Copyright © 2009 John Wiley & Sons, Ltd.