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In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts
Author(s) -
Chiang Huihua Kenny,
Peng FengYing,
Hung ShihChieh,
Feng YaChing
Publication year - 2009
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.2161
Subject(s) - mesenchymal stem cell , raman spectroscopy , stem cell , regenerative medicine , chemistry , in situ , microbiology and biotechnology , cellular differentiation , tissue engineering , biomedical engineering , biochemistry , biology , medicine , physics , organic chemistry , gene , optics
The differentiation of stem cells into specific cell types is playing an essential role in the development of stem cell therapy, tissue engineering, and regenerative medicine. In this research, Raman microspectroscopy was applied to monitor the development of hydroxyapatite [HA, Ca 5 (PO 4 ) 3 (OH)], which is associated with the differentiation of the human mesenchymal stem cells (hMSCs) into osteoblasts. Raman spectra exhibited dramatic changes in the HA region, 950–970 cm −1 , over the period of 7–21 days after the start of differentiation. This work demonstrates the successful application of Raman spectroscopy for monitoring and quantitatively evaluating hMSC differentiation into osteoblasts. Copyright © 2008 John Wiley & Sons, Ltd.