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Molecular vibrational imaging of a human cell by multiplex coherent anti‐Stokes Raman scattering microspectroscopy using a supercontinuum light source
Author(s) -
Kano Hideaki
Publication year - 2008
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.2041
Subject(s) - supercontinuum , raman scattering , raman spectroscopy , interphase , chemistry , nucleolus , biophysics , molecular physics , crystallography , optics , physics , biology , biochemistry , microbiology and biotechnology , optical fiber , photonic crystal fiber , cytoplasm
Molecular vibrational images of human cells are obtained by multiplex coherent anti‐Stokes Raman scattering (CARS) microspectroscopy. The CARS images of HeLa cells at the CH stretching vibrational mode clearly show various organelles such as nucleolus, chromosome, cell membrane, and nuclear membrane with high vibrational contrast. Filament‐like structures are also found to be observed in interphase, while it is not clearly observed in M phase. These structures most probably visualize endoplasmic reticulum (ER) network. Copyright © 2008 John Wiley & Sons, Ltd.