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Bridging biomolecules with nanoparticles: surface‐enhanced Raman scattering from colon carcinoma and normal tissue
Author(s) -
Pînzaru S. Cîntă,
Andronie L. M.,
Domsa I.,
Cozar O.,
Astilean S.
Publication year - 2008
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1907
Subject(s) - biomolecule , raman scattering , cytoplasm , nanoparticle , chemistry , raman spectroscopy , biophysics , laser , excitation , materials science , nanotechnology , biochemistry , optics , biology , physics , electrical engineering , engineering
In order to get insight into the chemical heterogeneities of solid tumors, here we report the first surface‐enhanced Raman scattering (SERS) experiment from normal and altered epithelial layer in human colon carcinoma tissues. The Ag colloidal nanoparticles that can be incorporated into the interstitial space in solid tumors or those penetrating into cytoplasm or nucleus of many cells allowed high quality SERS signal. Different tissue structures of tumor and normal colon have characteristic features in SERS spectra. Prominent SERS features of malignant tissue spectra are related to the strong enhancement of the bands preponderantly attributable to DNA or RNA bases. The preliminary studies demonstrate that it is possible to probe Ag colloidal nanoparticles adsorption onto the tissue resulting in a strong molecular signaling with high specificity and rapid acquisition time using visible laser line excitation. Copyright © 2008 John Wiley & Sons, Ltd.